MissingReferenceException Help Please!

MissingReferenceException: The object of type 'Image' has been destroyed but you are still trying to access it. So when I pick up an item for the first time it works okay, but after I reload the scene I get an issue where the item is not removed from the scene on pickup. It still appears in the players inventory however it also remains in the scene w$$anonymous$$ch doesn't look good.

I'll try and provide what info I can but please feel free to ask what else you want to see, I'm really trying to finish t$$anonymous$$s t$$anonymous$$ng!

So here is what's called when an item is picked up

     void Pickup()
     {
         Debug.Log ("Picking up" + item.name);
         bool wasPickedUp = InventoryManager.instance.Add (item);
         if (wasPickedUp) {
             gameObject.SetActive (false);
         }
     }