My sprite animation only flickers on Windows PC build

I've been working on a 2D project lately, because I'm a rookie in Unity so I chose a stupid way to present the animation of the ground, objects and all that in the scene: draw pictures one by one and put them into animations. So there are like thirtyish animations in my scene and played at the same time.

The problem is, all things worked fine in the editor, but once I build it into PC Windows exe, all my animations start flickering and glitching. I first thought it's because of the Layers, but I've already set them apart and even set a lot of settings such as sprite mip-map, Vsync, etc.

Recording of my problem: https://youtu.be/2CeEJ3oBZvU